The invention provides an antibacterial film. The antibacterial film is prepared from the following components in parts by mass: 1-8 parts of antibacterial peptide, 2-15 parts of gelatin, 0.5-5 partsof glycerol and water, wherein the concentration of antibacterial peptide is 0.10-0.35 g/ml; antibacterial peptide is produced from a lactobacillus paracasei subsp. Tolerans FX-6-1 strain by fermentation, lactobacillus paracasei subsp. Tolerans FX-6-1 is collected in China Center for Type Culture Collection on February 23, 2014 with collection number of CCTCCM 2014043. The antibacterial film has the beneficial effects that the antibacterial film belongs to active antibacterial package, and can better prevent water vapor from permeating, improve quality and safety of food and prolong the shelflife; besides, the antibacterial film has high tensile strength and elongation at break and wide application range, adopts a simple preparation method, and is suitable for large-scale production. Moreover, the antibacterial film material belongs to a food-grade raw material, and is easy to degrade and pollution-free to the environment.