
基本信息:
- 专利标题: 用于体外扩增牙髓干细胞的培养冻存体系、方法及试剂盒
- 专利标题(英):Culturing cryopreservation system, method and kit for amplifying dental pulp stem cells in vitro
- 申请号:CN201811584437.9 申请日:2018-12-24
- 公开(公告)号:CN109628389A 公开(公告)日:2019-04-16
- 发明人: 沈力坚 , 李明辉 , 王健 , 徐云龙 , 侯志勇 , 周书行 , 弓培 , 翟珊莉
- 申请人: 北京博奥晶典启衡生物科技有限公司
- 申请人地址: 北京市大兴区经济技术开发区科创六街88号院8号楼1单元301室
- 专利权人: 北京博奥晶典启衡生物科技有限公司
- 当前专利权人: 北京博奥晶典启衡生物科技有限公司
- 当前专利权人地址: 北京市大兴区经济技术开发区科创六街88号院8号楼1单元301室
- 代理机构: 北京知呱呱知识产权代理有限公司
- 代理人: 杜立军; 吴林
- 主分类号: C12N5/0775
- IPC分类号: C12N5/0775 ; A01N1/02
The embodiment of the invention discloses a culturing cryopreservation system for amplifying dental pulp stem cells in vitro. The culturing cryopreservation system comprises a dental pulp stem cellextracting reagent, a dental pulp stem cell culturing reagent and a dental pulp stem cell preservation reagent, and the culturing reagent comprises dental pulp stem cell original generation culturing liquid, dental pulp stem cell passage culturing agent anddental pulp stem cell digestive liquid. The embodiment of the invention further provides a culturing cryopreservation kit and method for amplifying the dental pulp stem cells in vitro. The culturing cryopreservation system for amplifying the dental pulp stem cells in vitro comprises all processes from preservation and conveying of the dental pulp stem cells in teeth to cryopreservation for the dental pulp stem cells. Storage of the dental pulp stem cells is simple in operation, and a large number of high-purity dental pulp cells can be obtained. The culturing cryopreservation system for amplifying the dental pulp stem cells in vitro reduces resource waste caused by preparation many times for different reagents and reduces the risk of cross contamination with other samples.