The invention provides an anti-fog coating and a preparation method thereof, and an anti-fog glass. The anti-fog coating comprises at least a cross-linking layer and at least an anti-fog layer, wherein the cross-linking layer and the anti-fog layer are alternately arranged to form the anti-fog coating, one side of the anti-fog coating is the cross-linking layer for being plated on a substrate, andthe other side is the anti-fog layer. According to the present invention, the anti-fog coating has advantages of long-lasting anti-fogging property, good wear resistance, good heat resistance and excellent water-solubility resistance; the preparation method of the anti-fog coating has advantages of scientific and reasonable steps, simple and easy-performing process, low cost and wide applicationrange; and the anti-fog glass can have excellent anti-fog effect while ensure the light transmittance.