The invention provides a method for accurately identifying disease resistance of newly-planted sugarcane white leaf. The method comprises: cutting sugarcane stalks infected with sugarcane white leaf phytoplasma, performing juicing, adding 10 times amount of sterile water to prepare an inoculating liquid; cutting the sugarcane stalks which are a sugarcane material to be tested into stalk sections with buds, soaking the sections in running tap water for 48h, performing hot water treatment at 50 DEG C for 2h, and performing soaking with an insecticidal and sterilizing agent for 10min; performingcoating inoculation with a plastic film, and performing moisture preservation at 25 DEG C for 24h; planting an inoculating material in a plastic barrel, and placing the barrel in a 20-30 DEG C insecticidal greenhouse for culture; and investigating infected plant rate, and performing disease resistance evaluation according to 1-5 grade standards. The method of the invention firstly establishes a set of accurate and efficient method for identifying disease resistance of newly-planted sugarcane white leaf, and provides technical support for sugarcane white leaf resistance breeding. The pathogen inoculating liquid is sprayed onto the sugarcane seed and the coating inoculation with a plastic film is performed, so that a disease habitat condition is provided, and the result is objective, true and reliable. The inoculation is quick and simple, the operability is strong, and the work efficiency is high. After the inoculation, the sugarcane white leaf has obvious incidence, high sensitivity andgood reproducibility.