The invention belongs to the field of medical instruments, and relates to a waterproof protection device for a male reproductive surgery and a manufacturing method of the waterproof protection device.The waterproof protection device comprises a waterproof protection device body, and a structure sealing a port of the waterproof protection device body is fused in the waterproof protection device body; the waterproof protection device body is composed of a front end, a clamping part, a body part, a trigger tensioning layer and a tail part, wherein the front end is connected with the clamping part, the clamping part is connected with the body part, the waterproof protection device body is made from a multilayer material, the clamping part is an edge protruding inward obliquely, the body partis in a bucket shape, the tail part is a sealing ring, and the trigger tensioning layer is arranged on the outer portion of a waterproof layer to achieve the whole water prevention of the waterproof protection device body and tightening of the waterproof protection device body to supply air to the inner portion of the waterproof protection device body. The waterproof protection device for the malereproductive surgery has the advantages that sealing can be achieved simply, conveniently and rapidly, the structure is simple, and water prevention is changed into sealing by using water during sealing, dynamic water prevention is achieved. The manufacturing method can perform more accurate and quicker manufacturing to achieve industrial production. The waterproof protection device for the malereproductive surgery and the manufacturing method thereof will have wide application in the field of the medical instruments.