The invention discloses a recombinant eukaryotic expression vector for preparing Xinjiang hemorrhagic fever medicine, a eukaryotic expression vector is obtain by combining a multi-epitope double-copygene fragment MEPX2 of Xinjiang hemorrhagic fever virus and an eukaryotic expression vector pVAX I, reasonable epitope design improves the immune efficacy and has the ability to focus the immune response on conservative epitopes, and it has good immunogenicity through the immunization of animal experiments. The recombinant plasmid was used as the antibody level of DNA vaccine combined with proteinvaccine. The cellular immunity level and immunity effect were better than single antigen immunization. The multi-epitope double-copy gene fragment MEPX2 of Xinjiang hemorrhagic fever virus strain YL04057 was recombined with eukaryotic expression vector pVAX I to form eukaryotic expression vector and immunized with protein vaccine, so overcome the shortcomings of poor immunity caused by single antigen, and have good development and application prospects.