The invention discloses an anti-accidental collision protection device of a high-low voltage distribution cabinet separation gate, which relates to the technical field of electric power equipment supporting facilities. The invention comprises a protective cover, a separating gate and a protective shell, wherein an opposite surface of the protective cover is provided with an air vent; an inherent clamping assembly of an opposing inner wall of the shield. The other end of the clamping assembly is clamped with the separating gate; a surface of that protective cover is provided with a first clamping groove; a surface of that shield is fixed with a plurality of first support blocks; a surface of that first support block is provided with a first through hole; a plurality of sliding grooves are arranged on the inner wall of the first through hole in a circular manner. The first through hole is rotatably connected with the protective housing through a circular shaft; a surface of that separation gate is provided with a switch; a surface of that separation gate is fixed with an indicator lamp; an opposite surface of the separation gate is provided with a second clamping groove. The invention prevents accidental collision to the separating gate through the protective cover and the protective shell, and adopts a clamping assembly to prevent the protective cover from falling off, and has the advantages of safety and reliability.