The invention discloses a preparation device of a novel antibacterial auxiliary agent. The preparation device comprises a support and a base plate which is fixedly mounted at the right bottom end of the support, wherein a fixed plate is mounted on a right end face of the support; a dovetail cavity is arranged in the right end face of the fixed plate; a dovetail block is mounted in the dovetail cavity in a sliding fit manner; a storage barrel is fixedly arranged on the right end face of the dovetail block; a carrying frame is arranged at the bottom end of the storage barrel; the carrying frameis fixedly arranged on a top end face of the base plate; a storage cavity is arranged in the top end face of the storage barrel; a discharging cavity is arranged at the right side of a bottom wall ofthe storage cavity in a manner of extending downward; an opening cavity is arranged in the discharging cavity in a communication manner; a baffle plate is mounted in the opening cavity in a rotary matching manner; a first electric rotation machine is mounted at the bottom end of the baffle plate in a power matching manner; the first electric rotation machine is fixedly arranged in the bottom wallof the opening cavity; and a rotary pipe barrel is mounted at the bottom end of the first electric rotation machine in the power matching manner; and a spline cavity is arranged in a bottom end face of the rotary pipe barrel.