The invention discloses an antibacterial release paper and a production method thereof. The antibacterial release paper comprises a base paper layer, a pre-coated layer and an isolation layer; the pre-coated layer comprises, by weight, 8-13 parts of lipoic acid, 6-15 parts of zinc hyaluronate, 4-8 parts of chitosan, 8-15 parts of seaweed gel, 5-10 parts of sodium lignosulfonate and 5-10 parts of butyl acetate; and the isolation layer comprises, by weight, 10-15 parts of an aqueous polyvinyl alcohol solution, 7-10 parts of benzotriazole, 5-8 parts of ferric citrate, 4-6 parts of polyoxyethylenecastor oil and 5-8 parts of disodium hydrogen phosphate. The antibacterial release paper is produced by compounding the base paper layer, the pre-coated layer and the isolation layer, and has the characteristics of good antibacterial effect and high strength, and the antibacterial rate of the antibacterial release paper on Escherichia coli and Staphylococcus aureus is 99% or more; and the production method has the advantages of easily available raw materials, easiness in realizing production process conditions, low production cost, and facilitation of production.