The invention discloses an application control method of a precise diagnosis and treatment device. The precise diagnosis and treatment device comprises an infusion bottle and an infusion tube, the infusion tube penetrates through a liquid-stopping valve body arranged on a fixing mechanism, the fixing mechanism comprises a fixed ring fixed at the head and a rotating ring rotatably arranged on an outer wall surface of the fixed ring, the liquid-stopping valve body is arranged on the rotating ring, a liquid level sensor is arranged in the infusion bottle, an infusion speed sensor and a flowmeterare arranged in the infusion tube, an inner wall surface of the fixed ring is connected with a fixing restraining belt, the inner side of the fixing restraining belt is connected with a cooling patch,the cooling patch is provided with a temperature sensor, the outer wall surface of the fixed ring is provided with a display screen for displaying the body temperature of a patient, the infusion speed and the infusion total flow, the liquid level sensor emits a signal to a controller if sensing that the liquid level in the infusion bottle is lower than a set safety liquid level position, and thecontroller controls the liquid-stopping valve body to start clamping and locking of the infusion tube. According to the method, the usage stability is good, the accuracy and the security are high, andthe application prospect is good.