The invention belongs to the technical field of health drinks, discloses an anti-cancer and anti-tumor ecological selenium-enriched fermented beverage. The beverage comprises organic ecological selenium powder, tea, antrodia, golden saffron, ash tree flowers, poria cocos, radix polygonati officinalis, truffle, cordyceps militaris, soybeans, tremellodongelatinosum, hericium erinaceus, honey fungus,shiitake mushrooms, apples, pears, bananas, oranges, carrot, tomatoes, brown sugar and water by mass. A preparation method comprises the steps of mixing: mixing all the materials in proportion; filling: putting the mixture into a high level tank and adding pure water at room temperature; fermentation: fermenting the materials for six months; filtration: removing residue, and leaving fermentationbroth only; sterilization: sterilizing the fermentation broth; and packaging: filling glass bottles with the fermentation broth, sealing and packaging. The beverage has anti-cancer, anti-tumor, anti-three-high, anti-oxidation and health care effects.