The invention discloses an assembly fixture and assembly method for a slot partition plate, and relates to an assembly fixture and assembly method for a partition plate. The problem of low assembly precision of an existing slot partition plate is solved. The assembly fixture is composed of a bottom plate, a plurality of partition plate inner ring pressure plates and a plurality of partition plateouter ring pressure plates; a main body of the bottom plate is in a disc shape, partition plate inner ring stop opening is formed in the upper surface of the bottom plate, a ring convex table is arranged on the outer side of the inner ring stop opening in the upper surface of the bottom plate, and a partition plate outer ring stop opening is formed in the upper surface of the ring convex table; and the partition plate inner ring pressure plates and the partition plate outer ring pressure plates are arranged on the upper surface of the bottom plate in a ring arraying mode. The assembly method comprises the steps that the inner ring of the partition plate is clamped into the inner ring stop opening, the outer ring of the partition plate is clamped into the outer ring stop opening, and a guide blade is installed between the inner ring and the outer ring of the partition plate for the brush and assembly of the partition plate guide blade. The brush and assembly of the partition plate guideblade are combined to one, the brush precision of the guide blade is improved, the assembly efficiency is improved, and the manufacturing cost is saved, and the assembly fixture is applicable to theassembly of the slot partition plate.