The invention discloses a multipoint positioning device for road and bridge construction engineering machine safety guarantee. The multipoint positioning device comprises a base, an opening is formedin the left end of the base, an inclined slope is arranged at the left end of the base, a groove where a roller is installed is formed in the middle portion of the upper end of the base, rocking handles are spirally connected to the two sides of the base and extend to the inner side of the base to be connected with gears, an engineering machine bearing base is arranged at the upper end of the base, and a bearing groove is formed in the middle portion of the upper end of the engineering machine bearing base. By means of the structure in the embodiment 1, transverse positioning box iron and a side plate are used for completing positioning and fixing of road and bridge construction engineering machines, and the road and bridge construction engineering machines are effectively prevented from moving to any positions in the using process; and by means of the structure in the embodiment 2, the road and bridge construction engineering machines are located at the position between parts of the structure, and the road and bridge construction engineering machines are effectively prevented from moving to any positions in the using process.