The invention provides an industrial production method of an intestinal tract probiotic preparation. The industrial production method comprises the following steps of (1) performing mass culture on lactobacillus casei, lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus acidophilus, streptococcus thermophilus, lactobacillus delbrueckii subspecies bulgaricus and the like through a fermenter; (2) performing centrifuging and washing with sterile water to obtain wet bacterium paste, and uniformly mixing the wet bacterium paste with a special protective agent in the ratio being 1 to 1;and (3) granulating a mixture of the wet bacterium paste and the protective agent, performing rolling to obtain balls, performing low-temperature vacuum drying, and performing coating so as to obtainthe intestinal tract probiotic preparation. Under the culture condition provided by the invention, probiotics can grow and propagate at high density, the collected wet bacterium paste does not need tobe subjected to freeze drying, the wet bacterium paste is directly subjected to vacuum low-temperature quick drying with the special protective agent, and massive bacterium bodies can be maintained to be alive; and energy resources are saved, and the production cost is reduced.