The embodiments of the invention disclose a network delay calculation method and device, equipment and a storage medium. The method comprises the steps of: determining, according to a virtual link architecture corresponding to a target network, a flow chart of a virtual link to be calculated in the target network, and determining a sequence link corresponding to the virtual link to be calculated and a non-sequence link according to the flow chart; removing the abnormal delay effect matched with the sequence link and caused by the concurrency from the flow chart, and then calculating an end-to-end delay upper limit value corresponding to the virtual link to be calculated for the flow chart by using a trajectory method. The technical solution solves the problems of low reliability, large deviation and little reference significance of AFDX (Avionics Full Duplex Switched Ethernet) end-to-end delay estimation results calculated in the prior art, thereby improving the reliability and reference value of the AFDX end-to-end delay evaluation results.