The invention discloses a deodorizing device of a livestock and poultry breeding shed. The deodorizing device comprises a breeding house, an oxygen generating device, a stirring tank and a water tank;the top of the breeding house is of a conical structure, the side wall of the breeding house is provided with an exhaust port, the breeding house is internally provided with a fixing transverse rod,and the lower end face of the fixing transverse rod is fixedly connected with a drug spraying device through connecting rods; the drug spraying device comprises a fixing plate, a column, a rotating motor and a nozzle and is connected with a gas conveying pipe and a liquid conveying pipe through connecting rods, the gas conveying pipe is fixedly connected with an output end of the oxygen generatingdevice, and the liquid conveying pipe is fixedly with a liquid outlet of the stirring tank; the stirring tank is internally provided with a stirring shaft, the stirring shaft is fixedly connected with an output end of a stirring motor, a microbial deodorant tank is fixedly installed at the top of the stirring tank, and a water inlet of the stirring tank is connected with the water tank through awater pipe. The deodorizing device is simple in structure and convenient to use, by spraying a mixture of microbial deodorant liquid and oxygen in the breeding house, the odor is removed, the effect is good, and the device is more environmentally friendly.