基本信息:
- 专利标题： 一种基于人铁蛋白的纳米酶及其制备方法
- 专利标题（英）：Nano-enzyme based on human ferritin and preparation method of nano-enzyme
- 申请号：CN201810470199.2 申请日：2018-05-16
- 公开(公告)号：CN108707618A 公开(公告)日：2018-10-26
- 发明人： 文继开 , 陈庆梅 , 张艳 , 邓诣群 , 吴骏 , 蒋珺 , 胡泰斗 , 胡章盛
- 申请人： 华南农业大学
- 申请人地址： 广东省广州市天河区五山路483号
- 专利权人： 华南农业大学
- 当前专利权人： 华南农业大学
- 当前专利权人地址： 广东省广州市天河区五山路483号
- 代理机构： 广州粤高专利商标代理有限公司
- 代理人： 单香杰
- 主分类号： C12N15/62
- IPC分类号： C12N15/62 ; C12N15/70 ; C12N9/16 ; C12N1/21 ; C12R1/19
The invention belongs to the technical field of gene engineering and particularly discloses a nano-enzyme based on human ferritin and a preparation method of the nano-enzyme. The preparation method includes the steps of adopting the human ferritin as a fusion partner, placing a target enzyme at an N end for fusion expression, and inserting a connecting peptide between the target enzyme and the human ferritin. The preparation method can remarkably improve the catalytic activity of the target enzyme on a substrate and enable the target enzyme to achieve better resistance to the temperature and pH. The preparation method is not only applicable to fusion expression between the human ferritin and parathion-methyl hydrolytic enzymes and fusion expression between the human ferritin and escherichia coli alkaline phosphatases but also suitable for fusion expression between the human ferritin and other diversified enzymes. The method is used for perfecting the features of the target enzyme and has a high research value and a scientific meaning on scientific research and basic research for application.
公开/授权文献:
- CN108707618B 一种基于人铁蛋白的纳米酶及其制备方法 公开/授权日：2022-04-08