The invention relates to a heat conduction structure with a liquid and gas separation mechanism. The structure comprises a temperature equilibrium plate, a heat pipe, a partition plate and work fluid;the temperature equilibrium plate comprises a shell and a containing cavity, the shell is provided with a bottom plate and a vertical plate, a first capillary structure is arranged on the inner surface of the bottom plate, a penetrating hole is formed in the vertical plate, the heat pipe comprises a pipe body and a second capillary structure, the pipe body is provided with the opening end, the opening end of the pipe body is in through-connection seal in a manner of corresponding to the penetrating hole, the partition board is laid on the opening end and covers the first capillary structure and the second capillary structure, a gas runner and a liquid runner are formed on the two sides of the partition board, and the containing cavity is filled with the work fluid. Backflow liquid-state work fluid cannot be affected by flowing of gas-state work fluid, and the backflow speed of the liquid-state work fluid is improved.