基本信息:
- 专利标题： 一种基因无痕编辑载体及其在生物体基因编辑中的应用
- 专利标题（英）：Gene traceless editing carrier and application of gene traceless editing carrier to organism gene editing
- 申请号：CN201810524747.5 申请日：2018-05-28
- 公开(公告)号：CN108676811A 公开(公告)日：2018-10-19
- 发明人： 郝志敏 , 曾凡力 , 胡玉笑 , 贾艳荣 , 赵相东 , 董金皋 , 申珅
- 申请人： 郝志敏 , 曾凡力 , 胡玉笑
- 申请人地址： 河北省保定市乐凯南大街2596号河北农业大学西校区B座2617室; ;
- 专利权人： 郝志敏,曾凡力,胡玉笑
- 当前专利权人： 河北福赛农业科技有限公司
- 当前专利权人地址： 河北省保定市乐凯南大街2596号河北农业大学西校区B座2617室; ;
- 代理机构： 北京汇信合知识产权代理有限公司
- 代理人： 赵倩
- 主分类号： C12N15/81
- IPC分类号： C12N15/81 ; C12N1/19 ; C12R1/865
The invention discloses a gene traceless editing carrier and application of the gene traceless editing carrier to organism gene editing. The gene traceless editing carrier consists of a genome homology sequence A, a promoter sequence, a cell wall protein CWP1 gene sequence, a resistance screening report gene sequence and a genome homology sequence B. By aiming at the problems that during the saccharomyces cerevisiae traditional gene knockout, screening markers are remained, the multigene knockout research is inconvenient, and the exogenous gene is remained, the invention provides a saccharomyces cerevisiae efficient gene traceless editing method. The method can be used for saccharomyces cerevisiae gene editing and can also be applicable to other microorganisms of the gene editing principle. The gene traceless editing carrier can be used for studying the function and metabolic mechanism of the yeast gene; no any exogenous gene is remained at an obtained mutant strain; the gene tracelessediting carrier can be safely used for industrial production.
公开/授权文献:
- CN108676811B 一种基因无痕编辑载体及其在生物体基因编辑中的应用 公开/授权日：2021-07-13