The invention discloses toltrazuril inclusion compound lyophilized powder. The toltrazuril inclusion compound lyophilized powder is obtained by adopting sulfobutyl ether-beta-cyclodextrin to perform inclusion on toltrazuril and then carrying out a lyophilization process. A preparation method of the toltrazuril inclusion compound lyophilized powder concretely comprises the following steps: dissolving the toltrazuril by an organic solvent to obtain an organic solution of the toltrazuril; adding the organic solution of the toltrazuril into an aqueous solution of the sulfobutyl ether-beta-cyclodextrin, and magnetically stirring for carrying out a reaction; carrying out rotary evaporation on the materials obtained after the reaction so as to remove the organic solvent, then filtering by using amicrofiltration membrane and drying to obtain a toltrazuril inclusion compound; carrying out the lyophilization process to obtain the toltrazuril inclusion compound lyophilized powder. According to the preparation method, the sulfobutyl ether-beta-cyclodextrin is adopted to perform inclusion on the toltrazuril, so that the solubility of the indissolvable drug-the toltrazuril is improved; by adopting the lyophilization process, the stability and bioavailability of the toltrazuril are improved; the toltrazuril inclusion compound lyophilized powder enriches the preparations of antiparasitic drugs.