基本信息:
- 专利标题： 胰内分泌细胞的制造方法和转分化剂
- 申请号：CN201680062837.6 申请日：2016-10-28
- 公开(公告)号：CN108431212A 公开(公告)日：2018-08-21
- 发明人： 松本征仁 , 岡崎康司 , 菅原泉
- 申请人： 学校法人顺天堂
- 申请人地址： 日本东京都
- 专利权人： 学校法人顺天堂
- 当前专利权人： 学校法人顺天堂
- 当前专利权人地址： 日本东京都
- 代理机构： 北京清亦华知识产权代理事务所
- 代理人： 宋融冰
- 优先权： 2015-212563 2015.10.29 JP
- 国际申请： PCT/JP2016/082095 2016.10.28
- 国际公布： WO2017/073740 JA 2017.05.04
- 进入国家日期： 2018-04-26
- 主分类号： C12N5/10
- IPC分类号： C12N5/10 ; C07K14/435 ; C12N5/071 ; C12N15/09
A method for producing pancreatic endocrine cells, said method comprising an introduction step in which any of the following genes or gene products are introduced into somatic cells: (A) a mutant GLIS1 gene or gene product thereof having at least 85% sequence identity with the nucleic acid sequence represented by SEQ. ID No. 1 or 2, a Neurogenin-3 gene or gene product thereof, a Pdx1 gene or gene product thereof, and a MafA gene or gene product thereof; (B) a mutant GLIS1 gene or gene product thereof having at least 85% sequence identity with the nucleic acid sequence represented by SEQ. ID No. 1 or 2, a Neurogenin-3 gene or gene product thereof, and a Pdx1 gene or gene product thereof; (C) a GLIS1 gene or gene product thereof, a Neurogenin-3 gene or gene product thereof, a Pdx1 gene or gene product thereof, and a MafA gene or gene product thereof; and (D) a mutant GLIS1 gene or gene product thereof having at least 85% sequence identity with the nucleic acid sequence represented by SEQ. ID No. 1 or 2, a Neurogenin-3 gene or gene product thereof, and a MafA gene or gene product thereof.
公开/授权文献:
- CN108431212B 胰内分泌细胞的制造方法和转分化剂 公开/授权日：2022-03-08