基本信息:
- 专利标题： 使用来自木质纤维材料的生化转化方法的固体残留物生产酶混合物的方法
- 申请号：CN201810161741.6 申请日：2012-11-27
- 公开(公告)号：CN108342332A 公开(公告)日：2018-07-31
- 发明人： F.本沙巴纳 , S.卢雷
- 申请人： IFP , 新能源公司
- 申请人地址： 法国吕埃-马迈松
- 专利权人： IFP,新能源公司
- 当前专利权人： IFP,新能源公司
- 当前专利权人地址： 法国吕埃-马迈松
- 代理机构： 中国专利代理(香港)有限公司
- 代理人： 李唐; 黄念
- 优先权： 11/03857 2011.12.14 FR
- 主分类号： C12N1/16
- IPC分类号： C12N1/16 ; C12N9/24 ; C12N9/42 ; C12P7/10 ; C12P21/00 ; C12R1/885 ; C12R1/66 ; C12R1/80 ; C12R1/645
The present invention concerns a process for the production of an enzymatic cocktail by submerged culture with a cellulolytic microorganism, comprising two phases: a phase a) for growth of said microorganism in the presence of at least one carbonaceous growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbonaceous growth substrate in the range 10 to 90 g/L; a phase b) for the production of the enzymatic cocktail, in which at least one carbonaceous inducer substrate is supplied, said carbonaceous inducer substrate being at least one solid residue obtained from the step for enzymatic hydrolysis of lignocellulosic materials which have undergone a pre-treatment step, said production phase being carried out with a concentration of carbonaceous production substrate in the range 150 to 400 g/L.