
基本信息:
- 专利标题: 有助于诱导生成神经前体细胞的诱导培养基
- 专利标题(英):Induction culture medium beneficial for inducing generation of neural precursor cells
- 申请号:CN201611101061.2 申请日:2016-12-05
- 公开(公告)号:CN108148808A 公开(公告)日:2018-06-12
- 发明人: 高绍荣 , 陈嘉瑜 , 高睿 , 张林凤 , 臧茹歌 , 王明珠 , 高亚威 , 陈珺
- 申请人: 同济大学 , 上海捷易生物科技有限公司
- 申请人地址: 上海市杨浦区四平路1239号
- 专利权人: 同济大学,上海捷易生物科技有限公司
- 当前专利权人: 同济大学,上海捷易生物科技有限公司
- 当前专利权人地址: 上海市杨浦区四平路1239号
- 代理机构: 上海沪慧律师事务所
- 代理人: 朱九皋
- 主分类号: C12N5/0797
- IPC分类号: C12N5/0797
The invention relates to an induction culture medium beneficial for inducing the generation of neural precursor cells. The induction culture medium contains the following ingredients with the corresponding contents: 87% DMEM/F12 culture medium (Gibco), 1% GlutaMAX (Gibco), 2% B27 minus vitamin A (Gibco), 1-10 [mu]g/mL heparin (Stem Cell Technologies), 1000 units/mL LIF (mLIF or hLIF) (Merck Millipore), 10-20 ng/mL basic fibroblast growth factor (bFGF; R&D Systems), and 10-40 ng/ml epidermal growth factor (EGF; Peprotech). The culture medium contains pedigree specific cell factors which are combined at a certain concentration. Through the induction culture medium, the mammal somatic cells can be induced to form neural precursor cells with functions under the in-vitro environment.
公开/授权文献:
- CN108148808B 有助于诱导生成神经前体细胞的诱导培养基 公开/授权日:2020-12-11