The invention discloses a real-time attendance check system based on two-dimensional codes and location service. The real-time attendance check system includes a user and right management module, an attendance check management module and a leave application management module; the user and right management module involves user application and registration, user login, user rights, user logout and password modification; the attendance check management module involves enabling attendance check, generating attendance check records, modifying attendance check records, and viewing attendance check records according to categories; and the leave application management module has four functions, namely uploading leave applications, modifying leave applications, previewing leave applications, and examining and approving leave applications. With the real-time attendance check system based on two-dimensional codes and location service of the present invention adopted, high-efficiency, automated and batched attendance check can be achieved, and high-efficiency, high-accuracy, and low-cost attendance check can be realized.