基本信息:
- 专利标题： 肝片吸虫多表位融合诊断抗原及其应用和制备方法
- 专利标题（英）：Fasciola hepatica multi-epitope fusion diagnosis antigen as well as application and preparation method thereof
- 申请号：CN201711340304.2 申请日：2017-12-14
- 公开(公告)号：CN108101974A 公开(公告)日：2018-06-01
- 发明人： 孟庆玲 , 乔军 , 王熙凤 , 乔梦凡 , 张凯 , 张国武 , 黄运福 , 孟丹 , 陈瑛 , 李重阳 , 贡莎莎 , 刘昱成
- 申请人： 石河子大学
- 申请人地址： 新疆维吾尔自治区石河子市北四路221号
- 专利权人： 石河子大学
- 当前专利权人： 石河子大学
- 当前专利权人地址： 新疆维吾尔自治区石河子市北四路221号
- 代理机构： 石河子恒智专利商标代理事务所
- 代理人： 李伯勤
- 主分类号： C07K14/435
- IPC分类号： C07K14/435 ; C12N15/70 ; G01N33/569 ; G01N33/68
The invention discloses a preparation method and the application of a fasciola hepatica multi-epitope fusion diagnosis antigen molecule Fh-meAg2, and belongs to the field of a biotechnology. Fh important genes SAP2, Cathepsin L1D and Cathepsin B4 are cloned, the amino acid sequence of the Fh important gene coding protein is analyzed by bioinformation software, dominant epitopes are screened, dominant antigen epitopes obtained through screening are connected in series by using flexible amino acid according to certain permutation and combination, and a multi-epitope fusion antigen (Fh-meAg) geneis established and synthesized. The Fh-meAg gene is cloned into an expression vector and expressed in Escherichia coli, the recombinant protein Fh-meAg is prepared, and the recombinant protein Fh-meAg2 with high reactogenicity is obtained through serologic test screening. Compared with the prior art, the fasciola hepatica multi-epitope fusion antigen Fh-meAg2 has high specificity and sensitivityand is an Fh infection diagnosis antigen molecule with application value.
公开/授权文献:
- CN108101974B 肝片吸虫多表位融合诊断抗原及其应用和制备方法 公开/授权日：2020-05-05
IPC结构图谱:
| C | 化学；冶金 |
| --C07 | 有机化学 |
| ----C07K | 肽 |
| ------C07K14/00 | 具有多于20个氨基酸的肽；促胃液素；生长激素释放抑制因子；促黑激素；其衍生物 |
| --------C07K14/435 | .来自动物；来自人类 |