![一种用于诱导多能干细胞的培养基及其方法](/CN/2017/1/234/images/201711170148.jpg)
基本信息:
- 专利标题: 一种用于诱导多能干细胞的培养基及其方法
- 专利标题(英):Culture medium for inducing multipotential stem cell and method for inducing multipotential stem cell
- 申请号:CN201711170148.X 申请日:2017-11-20
- 公开(公告)号:CN108004203A 公开(公告)日:2018-05-08
- 发明人: 彭特 , 陈勇 , 于云飞 , 蔡亚雄 , 刘樱 , 乔志平
- 申请人: 广东艾时代生物科技有限责任公司
- 申请人地址: 广东省佛山市顺德区乐从镇岭南大道南2号A栋7层717室
- 专利权人: 广东艾时代生物科技有限责任公司
- 当前专利权人: 广东艾时代生物科技有限责任公司
- 当前专利权人地址: 广东省佛山市顺德区乐从镇岭南大道南2号A栋7层717室
- 代理机构: 广州粤高专利商标代理有限公司
- 代理人: 陈卫
- 主分类号: C12N5/074
- IPC分类号: C12N5/074
The invention provides a culture medium for inducing a mltipotential stem cell and application thereof. The culture medium takes DMEM/F12 as a basal medium and comprises KSR, NEAA, Glutamine, beta-Mercaptoethanol, bFGF, a Rock inhibitor and an HDAC inhibitor. The culture medium for inducing the mltipotential stem cell does not contain animal serum, avoids the contamination of zoonotic pathogen, and improves the application safety. The inducing culture medium can reprogram the body cell to the mltipotential stem cell without a feed layer cell, and has the advantages of high efficiency, easinessin operation and the like. The obtained induced mltipotential stem cell can be stably sub-cultured, the karyotype is normal, and the mltipotential stem cell has the characteristic of multiple potentials.