The invention provides a biopharmaceutical constant temperature incubation device which comprises a supporting frame, supporting plates and a limit retainer ring. Four supporting rods are arranged atthe bottom of a box in a supporting manner, a roller is supported at the bottom of each supporting rod, eight supporting plates are annularly arrayed, welded and combined to form the supporting frame,a rectangular supporting sliding sleeve sleeves each supporting plate, four supporting clamp rods are symmetrically combined and supported to form a culture vessel placing rack, two culture vessel placing racks are arranged and supported at the top end of each supporting sliding sleeve, and culture vessels are slidably placed inside the culture vessel placing racks and supported and abutted to the top ends of the supporting sliding sleeves. Arc-surface sections are arranged on the inner sides of the supporting clamp rods, the four supporting clamp rods form each culture vessel placing rack, the device is applicable to slide assembly of circular disk culture vessels, the culture vessels are placed inside the culture vessel placing racks in a combined manner through slide assembly, and theoperation mode is simple and efficient.