基本信息:
- 专利标题： 一种用人尿液细胞作为饲养层培养诱导多能干细胞的方法
- 专利标题（英）：Method for culturing induced pluripotent stem cells by using human urine cells as feed layer
- 申请号：CN201711169918.9 申请日：2017-11-20
- 公开(公告)号：CN107937346A 公开(公告)日：2018-04-20
- 发明人： 于云飞 , 陈勇 , 彭特 , 蔡亚雄 , 刘樱 , 乔志平
- 申请人： 广东艾时代生物科技有限责任公司
- 申请人地址： 广东省佛山市顺德区乐从镇岭南大道南2号A栋7层717室
- 专利权人： 广东艾时代生物科技有限责任公司
- 当前专利权人： 广东艾时代生物科技有限责任公司
- 当前专利权人地址： 广东省佛山市顺德区乐从镇岭南大道南2号A栋7层717室
- 代理机构： 广州粤高专利商标代理有限公司
- 代理人： 陈卫
- 主分类号： C12N5/10
- IPC分类号： C12N5/10 ; C12N15/867
The invention provides a method for culturing human induced pluripotent stem cells by using human urine cells as a feed layer. Third-twelfth generations of human urine cells cultured after subculture,cryopreservation and resuscitation are used as trophoblast cells for culturing the human induced pluripotent stem (hiPS) cells reassembled by adult male foreskin fibroblasts. The used human urine cells replace small mouse embryo fibroblasts or mouse embryo fibroblast cell lines used in traditional methods, the human induced pluripotent stem cells are cultured, pollution of heterologous cells in the culture system is reduced, and the human induced pluripotent stem cells are more easily obtained than mesenchymal stem cells. The culture system is proved to make the human induced pluripotent stemcells amplified in vitro, the biological characteristics and the pluripotent property of the hiPS cells are maintained for a long time, and the possibility is provided for the hiPS to enter the clinical application.
公开/授权文献:
- CN107937346B 一种用人尿液细胞作为饲养层培养诱导多能干细胞的方法 公开/授权日：2021-02-19