The invention discloses a compound of keratinase and application in industrial production, and belongs to the field of the industrial biotechnology. The invention provides a method in which keratinaseis independently applied to the industrial tanning depilation or is compounded with lipase and amylase to be applied to the industrial tanning depilation, and the beneficial effects that depilation efficiency is high, the action conditions are mild, and the safety performance is good are achieved. The keratinase is adopted and can be used for independently completing sheepskin depilation, the quality of depilated leather is subjected to representation, and the depilation effect is the same as that of commercial enzyme; besides, lipase, amylase and keratinase are provided to perform compound depilation on sheepskin, the three kinds of enzymes are combined to have a collaborative effect on sheepskin depilation, the using amount of the enzymes can be reduced, and the depilation efficiency isimproved. The keratinase and a compounded enzyme preparation of the keratinase have good application prospects in the tanning field.