基本信息:
- 专利标题： 一种快速检测牛传染性鼻气管炎病毒的PCR扩增引物及其应用
- 专利标题（英）：PCR amplification primer for rapid detection of bovine infectious rhinotracheitis virus and applications thereof
- 申请号：CN201711130275.7 申请日：2017-11-15
- 公开(公告)号：CN107881259A 公开(公告)日：2018-04-06
- 发明人： 潘艳 , 李军 , 吴翠兰 , 彭昊 , 冯世文 , 陶立 , 李常挺 , 钟舒红 , 杨威 , 陈泽祥 , 谢永平 , 许力干 , 马春霞 , 胡帅 , 贺会利
- 申请人： 广西壮族自治区兽医研究所
- 申请人地址： 广西壮族自治区南宁市西乡塘区友爱北路51号
- 专利权人： 广西壮族自治区兽医研究所
- 当前专利权人： 广西壮族自治区兽医研究所
- 当前专利权人地址： 广西壮族自治区南宁市西乡塘区友爱北路51号
- 代理机构： 广州市红荔专利代理有限公司
- 代理人： 李彦孚; 何承鑫
- 主分类号： C12Q1/70
- IPC分类号： C12Q1/70 ; C12Q1/686 ; C12N15/11
The invention discloses a PCR amplification primer for the rapid detection of a bovine infectious rhinotracheitis virus and applications thereof, and belongs to the fields of animal bacteriology and molecular biology. The PCR amplification primer is composed of a forward primer having a nucleotide sequence represented by SEQ ID No.1 and a reverse primer having a nucleotide sequence represented bySEQ ID No.2. The PCR amplification primer is used to prepare a PCR amplification kit for detecting the bovine infectious rhinotracheitis virus. The provided PCR detection method using the kit has theadvantages of high specificity, high sensitivity, good repeatability, and high credibility. The bovine infectious rhinotracheitis virus can be detected specifically. The detection results can be obtained rapidly and accurately. At the same time, the cost is lower, the operation is simple, and the kit is suitable for being used in grassroots units; and a rapid, accurate and simple detection tool isprovided for laboratory rapid identification and large scale epidemiological investigation of bovine infectious rhinotracheitis virus.