The invention discloses a rotating and positioning operation worktable. The rotating and positioning operation worktable comprises a platform, a locking mechanism and a rotating column; the locking mechanism comprises a base, a first rotating arm and a second rotating arm; the base is fixed to the bottom of the platform, one end of the first rotating arm, and one end of the second rotating arm arehinged to the two ends of the base, the end, away from the base, of the second rotating arm is rotatably provided with a swing rod, the other end of the first rotating arm is connected with a swing rod through a screw nut, the inner side face of the first rotating arm and the inner side face of the second rotating arm are provided with teeth, the upper end of the rotating column is vertically provided with U-shaped tooth grooves allowing teeth to be inserted in along the circumference face of the rotating column, the upper end of the rotating column is rotatably connected to the platform located above the rotating column, and the rotating column is clamped between the first rotating arm and the second rotating arm. The platform fixedly provided with a non-standard tool can rotate relativeto the rotating column, through biting tooth engagement effect of the first rotating arm, the second rotating arm and the rotating column, the platform direction is locked, 360-degree rotation and positioning of the platform can be achieved, and the worktable is simple in structure, convenient, practical, and manual in operation.