The invention discloses a preparation method of a carrier by using saccharide and urea and Pt-Ni/NC catalyst with high performance for ethanol oxidation through hydrothermal synthesis on the carrier and a metal salt solution in a one-step method, and particularly relates to preparation of ethanol oxidation-electro-catalyst which is obtained by synthesizing sucrose (glucose) into a carbon carrier,adding the urea to realize doping of nitrogen, performing hydrothermal reaction and a metal salt solution and performing synthesis to obtain the catalyst, and then carbonizing the urea. The preparation method comprise the following main steps: firstly, adding the sucrose (glucose) and the urea into deionized water and then performing mixing to obtain a mixed solution; mixing a nickel chloride aqueous solution with a chloroplatinic acid aqueous solution according to a mass ratio to obtain a mixture; then adding the mixture into the mixed solution; regulating pH by using a sodium hydroxide aqueous solution, then performing ultrasonic treatment to enable the mixed solution to be evenly mixed; finally, putting into a polytetrafluoroethylene high-temperature high-pressure reaction kettle and carrying out hydrothermal reaction; cooling to a room temperature, then performing suction filtration and washing on the reacted solution to obtain a product; drying the obtained product in a vacuum drying box; carbonizing the obtained powder in a tubular furnace at a high temperature to obtain the spherical Pt-Ni/NC catalyst. Under an acidic condition, the Pt-Ni/NC catalyst prepared by the method disclosed by the invention has strong electro-catalytic oxidation activity and stability on ethanol, and the current density of the Pt-Ni/NC catalyst can reach 500mA.mg-1Pt; moreover, the Pt-Ni/NC catalyst is environmentally friendly and is free of pollution, and is relatively low in cost; therefore, the catalyst is a high-performance catalyst which can be directly applied to anodes of direct ethanol fuel cells.