The invention relates to a chicken sternal cartilage powder and a preparation method and application thereof. In the chicken sternal cartilage powder, the content of glucosamine is 10-15g/100g, the content of chondroitin sulfate is 1-2mg/100g, the content of hydroxyproline is 4-8g/100g, the content of basic fibroblast growth factor is 0.5-2.5mug/100g, and the content of insulin-like growth factor-1 is 0.1-0.5mg/100g; the preparation method comprises the following steps: taking fresh chicken sternal cartilage, completely removing non-cartilage matter, cleaning up and cutting into thin slices; freezing for at least 12-24 hours under the conditions below 60 DEG C, and freeze-drying; and then carrying out ultrafine grinding at a low temperature to obtain the chicken sternal cartilage powder. The preparation method of the chicken sternal cartilage powder provided by the invention is easy, convenient and feasible in technical scheme, strong in standardization, low in energy consumption, cost-saving and high in yield, and preserves the nutritional value of the chicken sternal cartilage to a greater extent.