The invention discloses a method for simulating mechanical stimulation of abnormal blood flow to calcification of valvular cells and relates to the technical field of biological model construction. The method comprises the following steps: S1) adopting a collagenase digestion method for extracting valvular interstitial cells in an aortic valve tissue and preparing into a cell supernatant culture medium; S2) mixing a magnetic bead with an RGD peptide stoste and preparing into a RGD peptide attached magnetic bead; S3) adding the RGD peptide attached magnetic bead into the cell supernatant culture medium acquired in the step S1), abandoning supernatant, cleaning with PBS and then adding into a first culture medium for culturing, putting the first culture medium into a magnetic distortion instrument, simulating the mechanical stimulation of the abnormal blood flow to the valvular interstitial cells by regulating the magnitude, frequency and acting time of the magnetic force of the magnetic distortion instrument and observing the calcification state of the valvular interstitial cells. According to the invention, the micro-simulation for the action of the mechanical stimulation of the abnormal blood flow to the calcification of valvular cells is realized, and meanwhile, the time for constructing a calcification model by a pure calcification culture medium can be shortened.