基本信息:
- 专利标题： 非-FRET肉毒梭菌测定法
- 专利标题（英）：Non-FRET botulinum assay
- 申请号：CN201710697371.3 申请日：2012-05-31
- 公开(公告)号：CN107475293A 公开(公告)日：2017-12-15
- 发明人： D.阿塔帕图 , W.塔克
- 申请人： 拜奥麦迪逊公司
- 申请人地址： 美国加利福尼亚州
- 专利权人： 拜奥麦迪逊公司
- 当前专利权人： 拜奥麦迪逊公司
- 当前专利权人地址： 美国加利福尼亚州
- 代理机构： 中国专利代理(香港)有限公司
- 代理人： 罗文锋
- 优先权： 61/492237 2011.06.01 US
- 主分类号： C12N15/85
- IPC分类号： C12N15/85 ; G01N33/569 ; G01N33/58 ; C07K19/00 ; C12N5/10
The invention relates to a non-FRET botulinum assay. A composition includes an artificial construct having (a) a reporter-containing portion chemically coupled to (b) a cleavage site. The cleavage site interacts with an investigational substance in a manner that cleaves the reporter-containing portion from a remainder of the construct. The cleaved portion is destroyed or otherwise degraded by the local environment, and presence of an investigational substance is evidenced by reduction in signal from the reporter. The investigational substance is preferably a Botulinum toxin (BoTN), and the cleavage sequence is all or part of a SNARE protein. The cleavable reporter-containing portion is preferably Yellow Fluorescent Protein (YFP), Citrine, Venus, or a YPet protein.