![一种高产纳豆激酶的液态培养基](/CN/2017/1/166/images/201710830145.jpg)
基本信息:
- 专利标题: 一种高产纳豆激酶的液态培养基
- 专利标题(英):Liquid culture medium for producing nattokinase in high yield
- 申请号:CN201710830145.8 申请日:2017-09-08
- 公开(公告)号:CN107475234A 公开(公告)日:2017-12-15
- 发明人: 龚爱华 , 朱沛煌 , 严永敏 , 曾建 , 彭琬昕 , 杜凤移 , 崔恒林
- 申请人: 江苏大学
- 申请人地址: 江苏省镇江市京口区学府路301号
- 专利权人: 江苏大学
- 当前专利权人: 江苏大学
- 当前专利权人地址: 江苏省镇江市京口区学府路301号
- 主分类号: C12N9/56
- IPC分类号: C12N9/56 ; C12R1/125
摘要:
本发明公开了一种高产纳豆激酶的液态培养基,属于生物技术领域。发酵培养基包括以下组分:红芸豆粉4.0%,葡萄糖4%,氯化钠1.5%,磷酸氢二钾0.05-0.2%,磷酸二氢钾0.05-0.2%,PH值7.0-7.4。与现有技术相比,该培养基组分简单,来源丰富,成本低廉,发酵纳豆激酶单位酶活力高,制作工艺简单,发酵液纳豆激酶活性达到33160u/ml。
摘要(英):
The invention discloses a liquid culture medium for producing nattokinase in high yield and belongs to the technical field of biology. A fermenting culture medium comprises the following components: 4.0% of red kidney bean powder, 4% of glucose, 1.5% of sodium chloride, 0.05-0.2% of dipotassium phosphate and 0.05-0.2% of monopotassium phosphate. The PH value is 7.0-7.4. Compared with the prior art, the culture medium has the advantages of simple components, abundant sources and low cost; the unit enzyme activity of the fermented nattokinase is high; the manufacturing technology is simple; the enzyme activity of the fermented nattokinase reaches up to 3160u/ml.