The patent discloses a device for shaking up a vacuum blood collection tube. The device comprises an engine base and a blood collection tube body, wherein the engine base is provided with a vertical rod, the vertical rod is provided with a sliding chute, a sliding block is connected in the sliding chute in a sliding manner, and a first pressure spring is arranged between the sliding block and an inner wall of the sliding chute; a clamping jaw is hinged on the sliding block; the top of the blood collection tube body is fixedly connected with an auxiliary block which is provided with a through hole; a sealing plate is arranged on the through hole, and two sides of the sealing plate are provided with spacing rings; the bottom of the sealing plate is symmetrically provided with fixing plates, an elastic iron piece is hinged on each fixing plate, and a first tension spring is arranged between each elastic iron piece and each fixing plate; two sides of the bottom of the blood collection tube body are hinged with arc-shaped plates; a second pressure spring is arranged between each arc-shaped plate and the blood collecting tube body; each arc-shaped plate is provided with a spacing groove; the engine base is further provided with a motor, a rotational shaft of the motor is provided with a circular shaft, a side wall of the circular shaft is hinged with a poke rod, and a second tension spring is arranged between the poke rod and the circular shaft. The scheme mainly solves the problem that medical workers suffer from hand muscle fatigue and strain caused by artificially shaking up the vacuum blood collection tube.