The present invention relates to a method for producing adherent cells according to which: a. adherent cells are injected into a culture container which contains microcarriers in a culture medium; b. the cells are amplified by performing a plurality of consecutive cell migrations in said same culture container, wherein each cell migration after the first cell migration is carried out: i. using all or part of the cell population obtained during the preceding cell migration, after having subjected said cell population to an enzymatic treatment in order to separate the cells from the microcarriers, and ii. by injecting culture medium and an increasing amount of microcarriers; and c. the cell population produced during the last cell migration is collected after having optionally subjected said cell population to an enzymatic treatment in order to separate the cells from the microcarriers. The invention also relates to the implementation of said method for the production of biological agents, in particular for preparing vaccines or drugs.