The invention discloses a wire harness winding machine. The wire harness winding machine comprises a machine frame, a rotating mechanism and a pulling mechanism, wherein the rotating mechanism and the pulling mechanism are mounted on the machine frame; the pulling mechanism is arranged on one side of the rotating mechanism and used for pulling wire harnesses; the rotating mechanism comprises a holder arranged at the top of the machine frame, a rotating wheel rotationally arranged on the holder, a limiting plate and a mounting seat; the limiting plate and the mounting seat are arranged on the rotating wheel; the two ends of the rotating wheel are cut-through; the two opposite plate surfaces of the limiting plate are provided with a limiting groove and a through groove correspondingly in the axis direction of the rotating wheel; the limiting groove communicates with the through groove; the mounting seat is located on the side, close to the through groove, of the limiting plate; and the mounting seat comprises a body provided with at least one groove in the side wall, a top bracing member arranged in the groove, and an elastic member elastically supported between the groove bottom of the groove and the top bracing member. The wire harness winding machine is high in product quality, capable of saving labor force, improving the work efficiency, convenient to use, high in practicability and suitable for application and popularization.