基本信息:
- 专利标题： 藏鸡过氧化物酶体增殖物激活受体α基因的SNP分子标记及应用
- 专利标题（英）：SNP (single nucleotide polymorphism) Molecular marker for peroxisome proliferator-activated receptor alpha gene in Tibetan chicken and application thereof
- 申请号：CN201710824000.7 申请日：2017-09-13
- 公开(公告)号：CN107365871A 公开(公告)日：2017-11-21
- 发明人： 钟成麟 , 赵小玲 , 舒刚 , 李思辰 , 刘嘉 , 李富贵 , 秦子越 , 覃飞 , 朱庆 , 王彦 , 尹华东
- 申请人： 四川农业大学
- 申请人地址： 四川省成都市温江区惠民路211号
- 专利权人： 四川农业大学
- 当前专利权人： 四川农业大学
- 当前专利权人地址： 四川省成都市温江区惠民路211号
- 代理机构： 北京科亿知识产权代理事务所
- 代理人： 汤东凤
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12N15/11
The invention discloses an SNP (single nucleotide polymorphism) molecular marker for peroxisome proliferator-activated receptor alpha gene in Tibetan chicken and application thereof. The SNP molecular marker is positioned at site T29467C of exon 4 of the peroxisome proliferator-activated receptor alpha gene in Tibetan chicken. A kit for detecting the SNP molecular marker provided herein comprises a primer having a nucleotide sequence shown as in SEQ ID No. 1-2. The invention also provides a method of using the SNP site to identify genotypes of Tibetan chicken; the method comprises: acquiring genome DNA of a chicken; using the primer pair having the nucleotide sequence shown as in SEQ ID No. 1-2 to detect the genotype of site T1289C; selecting individuals with the genotype CC. The results show that the PPAR (peroxisome proliferator-activated receptor) alpha gene exon 4N(T29467C) site allele has significant differences in Tibetan chicken and plain chicken; the mutated PPAR alpha better helps Tibetan chicken adapt to the high-altitude environment.