The invention belongs to the field of foodstuff processing, and concretely relates to a preparation method of marinated duck's feet. The preparation method comprises the following steps: monosodium glutamate, salt, monascus colour, Gynura bicolor, and tremella polysaccharide; auxiliary materials: soy and edible oil; the materials are boiled with water in order to prepare bittern; and then unfreezing with flowing water, trimming, blanching and residues removing, and frying and cooling of duck's feet are carried out; prefabricated bittern and duck's feet are placed in an interlayered pan, and boiling with big fire is carried out till boiling; the materials are boiled till boiling, steam in the interlayer pan is turned down, slow stewing with slow fire is carried out, finally the materials are taken out of the pan and cooled, and thickening with mixture of cornstarch and water is carried out; irradiation and sterilization are carried out, and weighing, packing and quick-freezing are carried out. The bittern can reduce smelling of fish or mutton in the duck's feet, and improve mouthfeel and nutritive values of duck's feet after long-term low temperature storage.