The invention discloses a preparation method of enzyme feed for cattle and sheep, made from 20 parts of mealworm enzyme liquid, 20 parts of fruit-vegetable enzyme liquid, 100 parts of straw powder, 40 parts of wheat flour, 40 parts of oat powder, 50 parts of bran powder, 30 parts of corn flour, 25 parts of potato flour, 10 parts of superfine mineral powder, 15 parts of superfine bone meal, 10 parts of egg white, 25 parts of peanut meal, 25 parts of rapeseed cake, 20 parts of seaweed powder, 10 parts of costus root, 3 parts of wheat bran, 5 parts of a vitamin complex, 5 parts of unsaturated fatty acid, 5 parts of edible salt, 12 parts of selenium-rich yeast, and 0.08 part of tea saponins. The invention also discloses a preparation method of enzyme feed for cattle and sheep. The enzyme feed for cattle and sheep can provide improved appetite for cattle and sheep, increased intake and improved disease resistance, can also provide increased selenium content in beef and mutton and reduced content of heavy metals in beef and mutton, and can provide significantly increased improved edible value of cattle and sheep and increased economic income for farmers.