基本信息:
- 专利标题： 一种基于ZmMIKC2a基因调节玉米籽粒淀粉含量的方法
- 专利标题（英）：Method for regulating content of starch in corn kernel based on ZmMIKC2a gene
- 申请号：CN201710533351.2 申请日：2017-07-03
- 公开(公告)号：CN107254475A 公开(公告)日：2017-10-17
- 发明人： 董庆 , 李廷春 , 王俊 , 王芳 , 张玮 , 陈洪俭 , 李成
- 申请人： 安徽省农业科学院烟草研究所
- 申请人地址： 安徽省合肥市农科南路40号
- 专利权人： 安徽省农业科学院烟草研究所
- 当前专利权人： 安徽省农业科学院烟草研究所
- 当前专利权人地址： 安徽省合肥市农科南路40号
- 代理机构： 北京华仁联合知识产权代理有限公司
- 代理人： 李珊
- 主分类号： C12N15/29
- IPC分类号： C12N15/29 ; C12N15/82 ; A01H5/10
The invention discloses a method for regulating the content of starch in a corn kernel based on a ZmMIKC2a gene and belongs to the technical field of gene engineering. The method comprises the following steps: I) constructing a ZmMIKC2a gene over-expression vector to obtain a ZmMIKC2a gene over-expression plant; II) knocking out the ZmMIKC2a gene in a target corn to obtain a ZmMIKC2a gene deletion plant. The method disclosed by the invention has the advantages that the positive regulation effect of the ZmMIKC2a gene on corn kernel starch synthesis related genes is utilized; the content of amylose in the target corn and the ratio of the amylase to total starch are improved or reduced through a gene engineering method, so that a new transgenic corn variety with high amylose, low amylopectin or high amylopectin/low amylose is obtained; the method is of great practical significance in developing the new transgenic corn variety with a specific function by utilizing a gene engineering technology.
公开/授权文献:
- CN107254475B 一种基于ZmMIKC2a基因调节玉米籽粒淀粉含量的方法 公开/授权日：2019-11-05