The invention discloses shrinkage-method sequential vitrification refrigerating fluid with an impervious protecting agent for oocyte and a usage method thereof. The sequential vitrification refrigerating fluid comprises shrinkage fluid, equilibrium fluid and vitrification fluid, wherein the shrinkage fluid is impervious protecting agent for dehydrating a cell. The shrinkage-method sequential vitrification refrigerating fluid has the advantages that (1), before entering the equilibrium fluid, the cell is firstly subjected to pretreatment by using the impervious protecting agent to be dehydrated; the acting time in the equilibrium fluid can be shortened; the chemical toxicity of the pervious protecting agent is decreased; (2), the impervious protecting agent is subjected to the pretreatment, so that the stability of the cytomembrane of an ovum can be improved; the oven is enabled to be more tolerant to an osmotic injury; the improvement of the survival rate of a resurgent oven is facilitated; (3), operators of different proficiency levels can all obtain a quite stable high survival rate; the long-time and large-sample-quantity technical training cannot need to be carried out before clinical application; (4), the result is stable; the operation is simple; the clinical wide popularization of a vitrification refrigeration technique for a human oocyte is greatly facilitated.