基本信息:
- 专利标题： 一种淀粉酶突变体制备方法及其应用
- 申请号：CN201710176535.8 申请日：2017-03-23
- 公开(公告)号：CN107201351B 公开(公告)日：2020-01-21
- 发明人： 姚斌 , 罗会颖 , 张多多 , 涂涛 , 王苑 , 黄火清 , 苏小运 , 柏映国 , 王亚茹
- 申请人： 中国农业科学院饲料研究所
- 申请人地址： 北京市海淀区中关村南大街12号
- 专利权人： 中国农业科学院饲料研究所
- 当前专利权人： 中国农业科学院北京畜牧兽医研究所
- 当前专利权人地址： 北京市海淀区中关村南大街12号
- 代理机构： 北京法信智言知识产权代理事务所
- 代理人： 刘静荣
- 主分类号： C12N9/30
- IPC分类号： C12N9/30 ; C12N15/81 ; C12N1/19 ; C12R1/84
The invention provides a high-specific-activity high-catalytic-efficiency amylase mutant and a preparation method and application thereof and relates to the field of gene engineering. Acid amylase derived from Talaromyces leycettanus JCM12802 is used as a female parent, and the wild type is subjected to site-directed mutagenesis by means of molecular biological techniques. Under the modification condition, the amylase mutant has significantly higher catalytic efficiency and specific activity than the wild (before mutation) female parent. By using the scheme, it is possible to greatly increase the specific activity and acting conditions of amylase, and basis is provided for the amylase in industrial production field. The scheme is of important guidance for increasing the catalytic efficiency and specific activity of amylase and other enzymes.
公开/授权文献:
- CN107201351A 一种高比活淀粉酶突变体制备方法及其应用 公开/授权日：2017-09-26