The invention belongs to the field of biological deodorizing, and discloses a deodorizing device and a deodorizing method using the same. The deodorizing device comprises a casing, a spraying assembly, a filler layer, an air distribution layer, a liquid storage layer, an air inlet and an air exhaust port, wherein the spraying assembly, the filler layer, the air distribution layer and the liquid storage layer are positioned in the casing and are sequentially arranged along the height direction of the casing from top to bottom; the air inlet is arranged on the casing and is communicated with the air distribution layer; the air exhaust port is arranged at the top part of the casing; the liquid storage layer comprises a liquid inlet and a liquid outlet, and the liquid outlet of the liquid storage layer is communicated together with the spraying assembly through a connecting pipeline; a circulating pump is arranged on the connecting pipeline, and the liquid inlet of the liquid storage layer is communicated with an external liquid source; the filler layer comprises microorganisms for deodorizing; when the liquid inlet of the liquid storage layer is closed, and the liquid outlet of the liquid storage layer, the air inlet of the circulating pump and the air exhaust port are opened, the deodorizing device starts a circulating, spraying and deodorizing mode. The deodorizing device disclosed by the invention can treat ozone containing high concentration of hydrogen sulfide gas at high efficiency.