基本信息:
- 专利标题： 表达载体元件组合、新的生产用细胞产生方法及其在重组产生多肽中的用途
- 专利标题（英）：Expression vector element combinations, novel production cell generation methods and their use for the recombinant production of polypeptides
- 申请号：CN201710324350.7 申请日：2012-12-19
- 公开(公告)号：CN107119073A 公开(公告)日：2017-09-01
- 发明人： P·M·许尔斯曼 , H·克内根
- 申请人： 弗·哈夫曼-拉罗切有限公司
- 申请人地址： 瑞士巴塞尔
- 专利权人： 弗·哈夫曼-拉罗切有限公司
- 当前专利权人： 弗·哈夫曼-拉罗切有限公司
- 当前专利权人地址： 瑞士巴塞尔
- 代理机构： 北京市中咨律师事务所
- 代理人： 凌立; 黄革生
- 优先权： 11195361.8 20111222 EP
- 主分类号： C12N15/85
- IPC分类号： C12N15/85 ; C12N15/13 ; C07K16/00 ; C07K16/28
Herein is reported that for transient transfections the use of the human elongation factor 1 alpha promoter (with Intron A) provides for an enhanced productivity (in LC-HC-SM organization), the use of the bovine growth hormone polyA signal sequence provides for an enhanced productivity compared to use of the SV40 polyA signal sequence, the addition of the HGT to the bGH PolyA signal sequence results in an increased productivity in vectors containing the hCMV promoter and the vector organization LC(3'-5')-HC-SM results in improved expression. For stable pools it is reported that pools generated with vectors containing the hEF1 alpha promoter show an enhanced productivity in batch analysis, clones generated with vectors containing the hEF1 alpha promoter show a reduced number of low producing clones, and clones generated with vectors containing the hEF1 alpha promoter show a higher stability of IgG expression. For single clones it is reported that the vector organization with downstream position of selection marker (LC-HC-SM) has a positive effect on productivity of single clones and that clones generated with vectors containing the bGH polyA signal sequence and the hGT have higher productivities.