The invention provides a hybridoma cell strain F-3-1 for secreting monoclonal antibodies for resisting total aflatoxins. The hybridoma cell strain is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.13806. The monoclonal antibodies for resisting total aflatoxins secreted by the cell strain has the advantages of high sensitivity, good specificity, and good anti-interference performance; the 50% inhibition concentration IC50 of aflatoxin B1 is 0.046[mu]g/L, cross reaction rate of the monoclonal antibodies and AFB2 is 110%, cross reaction rate of the monoclonal antibodies and AFG1 is 96%, and cross reaction rate of the monoclonal antibodies and AFG2 is 78%; the cell strain has good anti-interference performance for mycotoxin, pesticides, and heavy metals which may exist in traditional Chinese medicines, and accessories, contaminant microorganisms and the like used in processing, and provides a condition for immunodetection of total amount of aflatoxins B1, B2, G1 and G2 in traditional medicine with real application values.