The invention discloses a multi-channel polypeptide solid-phase synthesis device which comprises a reaction cylinder, wherein a suction filtration connecting pipe and a nitrogen connecting pipe are connected to the bottom of the reaction cylinder, the nitrogen connecting pipe is connected to a nitrogen hose, a nitrogen valve is arranged on the nitrogen connecting pipe, and a suction filtration valve is arranged on the suction filtration connecting pipe; the suction filtration connecting pipe is connected to a vacuumizing hose; a gas in-out pipe is arranged at the top of the reaction cylinder, a plurality of connecting ground openings are connected to the top surface of the reaction cylinder, a polypeptide synthesis pipe is inserted into each of the connecting ground openings, and the bottom end of the polypeptide synthesis pipe is inserted into the lower portion of the reaction cylinder and is provided with a sand core filter element; and a frosted connecting portion is arranged in the middle of the polypeptide synthesis pipe. By inserting different polypeptide synthesis pipes into the same reaction cylinder at the same time for batch reactions, compared with the prior art, the polypeptide solid phase synthesis efficiency is greatly increased, the corresponding research time is shortened, and various derived peptides can be quickly synthesized.