The invention discloses a ventilation cabinet for biomedical research experiments provided with a purification wall. The ventilation cabinet comprises a cabinet body; an operation chamber is formed in the cabinet body; a test bed is arranged at the bottom of the operation chamber; the purification wall is arranged on the back side wall of the operation chamber, and includes a cover body fixed on the back side wall of the operation chamber; a frame is arranged in the cover body, and is provided with an activated carbon output box, an activated carbon recovery box and a vacuum cavity; a cage is fixed in the frame; the front side wall and the back side wall of the cage respectively lean against front fiber cloth and back fiber cloth; the cage, the front fiber cloth and the back fiber cloth form a filter cavity; activated carbons are filled in the filter cavity; the activated carbon output box is corresponding to the upper end of the filter cavity; and the lower end of the filter cavity is corresponding to the activated carbon recovery box. The ventilation cabinet can further purify air in the operation chamber to improve the cleanness in the operation chamber, and can automatically replace activated carbons.